Enzyme Immobilization: An Overview on Nanoparticles as Immobilization Matrix
نویسندگان
چکیده
Immobilization process is to optimize the operational performance of an enzyme for industrial applications. So far different matrices have been described in the literature to improve the performance of the immobilized enzymes. With the advent of nanotechnology, the nanomaterials because of their unique physico-chemical properties constitute novel and interesting matrices for enzyme immobilization. The nanomaterials possess ideal characteristics to equilibrate principal factors which determine biocatalysts efficiency, including specific surface area, mass transfer resistance and effective enzyme loading. This review presents the current scenario and techniques in enzyme immobilization. An overview of the main methods used to combine proteins/enzymes with nanoparticles is given in the study. The advantages and disadvantages of nanoparticles as immobilization matrix are also discussed.
منابع مشابه
Immobilization of the Alkaline Phosphatase on Collagen Surface via Cross-Linking Method
Background: Collagen, the most abundant protein in the human body, and as an extracellular matrix protein, has an important role in the fiber formation. This feature of the collagen renders establishment of the structural skeleton in tissues. Regarding specific features associated with the collagen, such as, formation of the porous structure, permeability and hydrophilicity, it can also be use...
متن کاملEffects of cadmium chloride as inhibitor on stability and kinetics of immobilized Lactoperoxidase(LPO) on silica-coated magnetite nanoparticles versus free LPO
Objective(s): Enzyme immobilization via nanoparticles is perfectly compatible against the other chemical or biological approximate to improve enzyme functions and stability. In this study lactoperoxidase was immobilized onto silica-coated magnetite nanoparticles to improve enzyme properties in the presence of cadmium chloride as an inhibitor. Materials and Methods: The process consists of the ...
متن کاملThe immobilization of laccase enzyme from Trametes versicolor on the surface of porous zinc oxide nanoparticles and studying features of the immobilized enzyme
The laccase enzyme is the largest group of Oxidoreductase enzymes and is capable of oxidizing a wide range of organic substrates to water along with molecular oxygen resuscitation. ZnO nanoparticles are known for their specific properties such as chemical stability, high electrochemical coupling rates, and wide range of absorption of radiation as multifunctional compounds. In this study, ZnO po...
متن کاملThe immobilization of laccase enzyme from Trametes versicolor on the surface of porous zinc oxide nanoparticles and studying features of the immobilized enzyme
The laccase enzyme is the largest group of Oxidoreductase enzymes and is capable of oxidizing a wide range of organic substrates to water along with molecular oxygen resuscitation. ZnO nanoparticles are known for their specific properties such as chemical stability, high electrochemical coupling rates, and wide range of absorption of radiation as multifunctional compounds. In this study, ZnO po...
متن کاملInvestigation of Enzyme Immobilization Effects on its Characteristics
Background; Enzymes are well known as sensitive catalysts in the laboratory and industrial scale. To improve their properties and for using their significant potential in various reactions as a useful catalyst the stability of enzymes can often require improvement. Enzymes Immobilization on solid supports such as epoxy- functionalized ferric silica nanocomposite can be effective way to improve ...
متن کامل